Arabinoxylan rice bran (MGN-3/Biobran) alleviates radiation-induced intestinal barrier dysfunction of mice in a mitochondrion-dependent manner PEER REVIEWED
- Zhenguo Zhao , Wei Chengb, Wei Qu , Kai Wang
- (edited by Chris Gutch PhD.)
- 2020
MGN-3 is an arabinoxylan from rice bran that has been shown to be an excellent antioxidant and radioprotector.
This study examined the protective effects of MGN-3 on radiation-induced intestinal injury. Mice were treated
with MGN-3 prior to irradiation, then continued to receive MGN-3 for 4 weeks thereafter. MGN-3 increased the
activity of mitochondrial respiratory chain complexes Ⅰ, Ⅲ, Ⅳ and Ⅴ, the intercellular ATP content, the mitochondria-encoded gene expression and mitochondrial copy numbers in the jejunal and colonic mucosa. MGN-3
reduced the oxidative stress levels and inflammatory response indicators in the serum and jejunal and colonic
mucosa. Antioxidant indicators such as superoxide dismutase, glutathione peroxidase, catalase and total antioxidant capacity were significantly increased in the serum and jejunal and colonic mucosa in the MGN-3 group.
Moreover, MGN-3 decreased the gene abundances and enzymatic activities of caspase-3, 8, 9 and 10 in the
jejunal and colonic mucosa. The endotoxin, diamine peroxidase, D-lactate and zonulin levels were significantly
reduced in the serum and jejunal and colonic mucosa in the MGN-3 group. MGN-3 also markedly upregulated the
gene abundances of ZO-1, occludin, claudin-1 and mucin 2. MGN-3 effectively attenuated radiation-induced
changes in the intestinal epithelial mitochondrial function, oxidative stress, inflammatory response, apoptosis,
intestinal permeability and barrier function in mice. These findings add to our understanding of the potential
mechanisms by which MGN-3 alleviates radioactive intestinal injury.